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nuclear marker dapi  (Beyotime)


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    Structured Review

    Beyotime nuclear marker dapi
    Nuclear Marker Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 31805 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nuclear+marker+dapi/DAPI/pm40250529-117-16-20
    Average 99 stars, based on 31805 article reviews
    nuclear marker dapi - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Marker:

    Article Title: Overexpression of S100B promotes depressive-like behaviors in stroke-induced rats by modulating the PI3K/AKT/NF-κB pathway.
    Article Snippet: Post-stroke depression (PSD) is a common complication following a stroke, primarily characterized by low mood, cognitive sluggishness, and sleep disturbances.. Currently, the precise pathogenic mechanisms underlying PSD remain elusive.. Research indicates that S100B protein levels may serve as a specific biochemical marker of organic brain injury, with significantly elevated serum S100B levels noted in patients with ischemic stroke, depression, and schizophrenia.

    Article Title: Multi-omics revealed activation of TNF-α induced apoptosis signaling pathway in testis of DEHP treated prepubertal male rat.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: DAPK1 mediates cognitive dysfunction and neuronal apoptosis in PSD rats through the ERK/CREB/BDNF signaling pathway.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: The gut metabolite 3-hydroxyphenylacetic acid rejuvenates spermatogenic dysfunction in aged mice through GPX4-mediated ferroptosis
    Article Snippet: After washing in PBS for three times, tissues were incubated with the following secondary antibodies at room temperature for 1 h: Cy3-conjugated AffiniPure donkey polyclonal anti-mouse IgG (H + L) and Alexa Fluor 488-conjugated AffiniPure donkey polyclonal anti-rabbit IgG (H + L) (1:500; Jackson ImmunoResearch Laboratories, Philadelphia, PA, USA). .. The tissues were counterstained with the nuclear marker DAPI (100 ng/mL, Beyotime, Jiangsu, China) carrying blue fluorescence for 10 min at room temperature. .. After three washes in PBS, the slides were mounted in Gel-Mount medium and observed under a confocal microscope (Zeiss LSM710, Carl Zeiss Microscopy GmbH, Jena, Germany) at excitation wavelengths of 488 nm (green), 555 nm (red), and 405 nm (blue).

    Article Title: The gut metabolite 3-hydroxyphenylacetic acid rejuvenates spermatogenic dysfunction in aged mice through GPX4-mediated ferroptosis.
    Article Snippet: After washing in PBS for three times, tissues were incubated with the following secondary antibodies at room temperature for 1 h: Cy3-conjugated AffiniPure donkey polyclonal anti-mouse IgG (H + L) and Alexa Fluor 488-conjugated AffiniPure donkey polyclonal anti-rabbit IgG (H + L) (1:500; Jackson ImmunoResearch Laboratories, Philadelphia, PA, USA). .. The tissues were counterstained with the nuclear marker DAPI (100 ng/ mL, Beyotime, Jiangsu, China) carrying blue fluorescence for 10 min at room temperature. .. After three washes in PBS, the slides were mounted in Gel-Mount medium and observed under a confocal microscope (Zeiss LSM710, Carl Zeiss Microscopy GmbH, Jena, Germany) at excitation wavelengths of 488 nm (green), 555 nm (red), and 405 nm (blue).

    Article Title: Roles of ferroptosis in type 1 diabetes induced spermatogenic dysfunction.
    Article Snippet: Diabetes mellitus (DM) is a widespread metabolic disease presenting with various complications, including spermatogenic dysfunction.. However, the underlying mechanisms are still unclear.. Ferroptosis, a novel type of programmed cell death, is associated with much metabolic diseases.

    Article Title: High-frequency repetitive transcranial magnetic stimulation improves depressive-like behaviors in CUMS-induced rats by modulating astrocyte GLT-1 to reduce glutamate toxicity.
    Article Snippet: Impaired glutamate recycling plays an important role in the pathophysiology of depression, and it has been demonstrated that glutamate transporter-1 (GLT-1) on astrocytes is involved in glutamate uptake.. Studies have shown that repetitive transcranial magnetic stimulation (rTMS) is effective in treating depression, however, the exact mechanism of rTMS treatment remains unclear.. Here, we used a chronic unpredictable mild stress (CUMS) protocol to induce depression-like behaviors in rats followed by rTMS treatment.

    Article Title: Novel Amphibian Bowman-Birk-Like Inhibitor with Antioxidant and Anticoagulant Effects Ameliorates Pancreatitis Symptoms in Mice.
    Article Snippet: Acute pancreatitis (AP) is a serious inflammatory disorder and still lacks effective therapy globally.. In this study, a novel Ranacyclin peptide, Ranacin, was identified from the skin of Pelophylax nigromaculatus frog.. Ranacin adopted a compact βhairpin conformation with a disulfide bond (Cys5−Cys15).

    Fluorescence:

    Article Title: Multi-omics revealed activation of TNF-α induced apoptosis signaling pathway in testis of DEHP treated prepubertal male rat.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: The gut metabolite 3-hydroxyphenylacetic acid rejuvenates spermatogenic dysfunction in aged mice through GPX4-mediated ferroptosis
    Article Snippet: After washing in PBS for three times, tissues were incubated with the following secondary antibodies at room temperature for 1 h: Cy3-conjugated AffiniPure donkey polyclonal anti-mouse IgG (H + L) and Alexa Fluor 488-conjugated AffiniPure donkey polyclonal anti-rabbit IgG (H + L) (1:500; Jackson ImmunoResearch Laboratories, Philadelphia, PA, USA). .. The tissues were counterstained with the nuclear marker DAPI (100 ng/mL, Beyotime, Jiangsu, China) carrying blue fluorescence for 10 min at room temperature. .. After three washes in PBS, the slides were mounted in Gel-Mount medium and observed under a confocal microscope (Zeiss LSM710, Carl Zeiss Microscopy GmbH, Jena, Germany) at excitation wavelengths of 488 nm (green), 555 nm (red), and 405 nm (blue).

    Article Title: The gut metabolite 3-hydroxyphenylacetic acid rejuvenates spermatogenic dysfunction in aged mice through GPX4-mediated ferroptosis.
    Article Snippet: After washing in PBS for three times, tissues were incubated with the following secondary antibodies at room temperature for 1 h: Cy3-conjugated AffiniPure donkey polyclonal anti-mouse IgG (H + L) and Alexa Fluor 488-conjugated AffiniPure donkey polyclonal anti-rabbit IgG (H + L) (1:500; Jackson ImmunoResearch Laboratories, Philadelphia, PA, USA). .. The tissues were counterstained with the nuclear marker DAPI (100 ng/ mL, Beyotime, Jiangsu, China) carrying blue fluorescence for 10 min at room temperature. .. After three washes in PBS, the slides were mounted in Gel-Mount medium and observed under a confocal microscope (Zeiss LSM710, Carl Zeiss Microscopy GmbH, Jena, Germany) at excitation wavelengths of 488 nm (green), 555 nm (red), and 405 nm (blue).

    Article Title: Roles of ferroptosis in type 1 diabetes induced spermatogenic dysfunction.
    Article Snippet: Diabetes mellitus (DM) is a widespread metabolic disease presenting with various complications, including spermatogenic dysfunction.. However, the underlying mechanisms are still unclear.. Ferroptosis, a novel type of programmed cell death, is associated with much metabolic diseases.



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    Expression of key genes at mRNA and protein levels in the hippocampus of TLE animal model. (A) Construction of the TLE mouse model. It primarily illustrates the process of animal anesthesia and fixation, kainic acid injection localization, brain tissue separation on ice, and hippocampus extraction. (B) Expression levels of core genes' corresponding mRNA in the hippocampus of NC and TLE groups identified by qPCR. Data are shown as mean ± SE. (C) Western blot analysis of hippocampal lysates displaying the protein levels of Dnmt1, Cdc25b, Ssh2, Fgd3, Gzma, Raf1, and Mx1 with Actin serving as a loading control. <t>Approximate</t> <t>molecular</t> weights are indicated. (D) Quantification of Western blot analysis of the protein bands in Figure C, through relative gray values compared across NC and TLE hippocampus samples. (E) Quantitative analyses of immunofluorescence staining showing the fluorescence intensity between NC and TLE in Figure F. (F) Representative immunofluorescence images showing the hippocampal localization of Cdc25b, Raf1, Fgd3, Dnmt1, Mx1, and Ssh2 (red) with <t>DAPI</t> staining the nuclei (blue) in NC and TLE groups (Scale bar, 100 μm). (TLE, temporal lobe epilepsy; NC, normal control. * p < 0.05, ** p < 0.01, *** p < 0.001, ns, not significant.)
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    Image Search Results


    Expression of key genes at mRNA and protein levels in the hippocampus of TLE animal model. (A) Construction of the TLE mouse model. It primarily illustrates the process of animal anesthesia and fixation, kainic acid injection localization, brain tissue separation on ice, and hippocampus extraction. (B) Expression levels of core genes' corresponding mRNA in the hippocampus of NC and TLE groups identified by qPCR. Data are shown as mean ± SE. (C) Western blot analysis of hippocampal lysates displaying the protein levels of Dnmt1, Cdc25b, Ssh2, Fgd3, Gzma, Raf1, and Mx1 with Actin serving as a loading control. Approximate molecular weights are indicated. (D) Quantification of Western blot analysis of the protein bands in Figure C, through relative gray values compared across NC and TLE hippocampus samples. (E) Quantitative analyses of immunofluorescence staining showing the fluorescence intensity between NC and TLE in Figure F. (F) Representative immunofluorescence images showing the hippocampal localization of Cdc25b, Raf1, Fgd3, Dnmt1, Mx1, and Ssh2 (red) with DAPI staining the nuclei (blue) in NC and TLE groups (Scale bar, 100 μm). (TLE, temporal lobe epilepsy; NC, normal control. * p < 0.05, ** p < 0.01, *** p < 0.001, ns, not significant.)

    Journal: CNS Neuroscience & Therapeutics

    Article Title: Integrated Mendelian Randomization and Single‐Cell Transcriptomics Analysis Identifies Critical Blood Biomarkers and Potential Mechanisms in Epilepsy

    doi: 10.1111/cns.70172

    Figure Lengend Snippet: Expression of key genes at mRNA and protein levels in the hippocampus of TLE animal model. (A) Construction of the TLE mouse model. It primarily illustrates the process of animal anesthesia and fixation, kainic acid injection localization, brain tissue separation on ice, and hippocampus extraction. (B) Expression levels of core genes' corresponding mRNA in the hippocampus of NC and TLE groups identified by qPCR. Data are shown as mean ± SE. (C) Western blot analysis of hippocampal lysates displaying the protein levels of Dnmt1, Cdc25b, Ssh2, Fgd3, Gzma, Raf1, and Mx1 with Actin serving as a loading control. Approximate molecular weights are indicated. (D) Quantification of Western blot analysis of the protein bands in Figure C, through relative gray values compared across NC and TLE hippocampus samples. (E) Quantitative analyses of immunofluorescence staining showing the fluorescence intensity between NC and TLE in Figure F. (F) Representative immunofluorescence images showing the hippocampal localization of Cdc25b, Raf1, Fgd3, Dnmt1, Mx1, and Ssh2 (red) with DAPI staining the nuclei (blue) in NC and TLE groups (Scale bar, 100 μm). (TLE, temporal lobe epilepsy; NC, normal control. * p < 0.05, ** p < 0.01, *** p < 0.001, ns, not significant.)

    Article Snippet: Subsequently, slices were briefly rinsed in PBS for 5 min and stained with the DAPI nuclear marker (# D1306, 1:10000, Molecular Probes) for 5 min. After a final PBS rinse, slices were mounted on gel‐coated slides, cover‐slipped with Prolong gold anti‐fade (# P36930, Vector Laboratories), and stored at 4°C until further analysis.

    Techniques: Expressing, Animal Model, Injection, Extraction, Western Blot, Control, Immunofluorescence, Staining, Fluorescence